WebAssay Principle Principle of the plug and play GFP Transfection Efficiency Assay using the NucleoCounter® NC-3000™: Cells are located using Hoechst 33342 (blue) and the percentage of GFP expressing cells (green) can easily be determined. Non-viable cells are stained with propidium iodide (PI; red) WebJul 13, 2024 · GFP disruption assay. HEK293T cells were first lentivirally transduced with a destabilized GFP (d2GFP) reporter (derived from Addgene #14760).
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WebJan 21, 2024 · Fluorescent protein disruption assay Fluorescent protein disruption assays were performed to evaluate DNA cleavage and indel-mediated disruption at the target site of the fluorescent protein (i.e. GFP) brought by SaCas9 and gRNA expressions, which results in loss of cell fluorescence. Web(E) GFP disruption assay: HEK293 cells were transfected with cLNPs at different concentrations (0.1 to 1 g/ml, 0.7 to 7 nM total RNA), and 72 hours after transfection, the percentage of GFP +... projected interest rates 2023 australia
GFP Transfection Efficiency Assay - NucleoCounter® Image …
WebFeb 23, 2024 · In the case of the ABTS+ assay, it was shown that this radical is most strongly scavenged by A. melanocarpa followed by R. nigrum and V. myrtillus (Table 5). In comparison, the IC 50 value of ascorbic acid was 11.8 µg/mL ± 0.06 in the DPPH assay and 9.3 µg/mL ± 0.04 in the ABTS assay, respectively. The results of both tests clearly … WebSep 24, 2024 · We used the proportion of GFP-negative cells to estimate the gene disruption rates of each nuclease, although the former may have been lower than the … WebForced expression of SOCS1 attenuates LPS-induced endothelial barrier disruption and inflammation. A ... EB1 or β-tubulin and Myc-SOCS1 were tested in co-IP assay with GFP antibody followed by immunoblotting with Myc-antibody. Probing of total cell lysates with GFP and Myc antibodies was used to verify ectopic protein expression; membrane re ... lab created teal sapphire